Identification of SNPs in RNA-seq data of two cultivars of Glycine max (soybean) differing in drought resistance
نویسندگان
چکیده
The legume Glycine max (soybean) plays an important economic role in the international commodities market, with a world production of almost 260 million tons for the 2009/2010 harvest. The increase in drought events in the last decade has caused production losses in recent harvests. This fact compels us to understand the drought tolerance mechanisms in soybean, taking into account its variability among commercial and developing cultivars. In order to identify single nucleotide polymorphisms (SNPs) in genes up-regulated during drought stress, we evaluated suppression subtractive libraries (SSH) from two contrasting cultivars upon water deprivation: sensitive (BR 16) and tolerant (Embrapa 48). A total of 2,222 soybean genes were up-regulated in both cultivars. Our method identified more than 6,000 SNPs in tolerant and sensitive Brazilian cultivars in those drought stress related genes. Among these SNPs, 165 (in 127 genes) are positioned at soybean chromosome ends, including transcription factors (MYB, WRKY) related to tolerance to abiotic stress.
منابع مشابه
THE EFFECT OF DROUGHT STRESS ON GROWTH, PHOTOSYNTHETIC FACTORS, CONTENT OF PROTEIN, Na AND K CONTENT IN TWO CULTIVARS OF SOYBEAN
In this research, the response of shoot and root of two cultivars of soybean (Glycine max L. cvs Gorgan 3 & Williams) to mild and severe drought was evaluated in pot. Two weeks after of planting, three irrigation treatments including every day (as control), once in three (as mild stress) and five days (as severe stress) were used. The results showed that, stem dry weight, stem and root length, ...
متن کاملIdentification of soybean circular RNAs in response to low nitrogen and phosphorus stress
Soybean, one of the most important sources of edible oil and protein in the world, is exposed to various environmental biotic and abiotic stresses. These stresses can negatively impact the quality and quantity of soybean production. This study aimed to identify genes that express circular RNAs in response to low phosphorus and nitrogen stresses in soybean roots. Soybean seeds were grown under d...
متن کاملEffect of UV-B radiation on photosynthetic pigments and UV-absorbing compounds of three different soybean cultivars (Glycine max L.).
The effects of UV-B radiation on the amount of chlorophyll a, b, carotenoids, flavonoids, anthocyanins, and polyphenols of three soybean cultivars was studied. After germination in the incubator, plants were put in pots of soil and during 3 weeks and irrigated with Hoagland solution every day. After 3 weeks, plants were exposed to UV-B for 20 min every day for a week. In each cultivar, UV-B red...
متن کاملRNA-seq data comparisons of wild soybean genotypes in response to soybean cyst nematode (Heterodera glycines)
Soybean [Glycine max (L.) Merr.] is an important crop rich in vegetable protein and oil, and is a staple food for human and animals worldwide. However, soybean plants have been challenged by soybean cyst nematode (SCN, Heterodera glycines), one of the most damaging pests found in soybean fields. Applying SCN-resistant cultivars is the most efficient and environmentally friendly strategy to mana...
متن کاملAssessment of seed storage protein composition of six Iranian adopted soybean cultivars [Glycine max (L.) Merrill.]
Seed protein quality is an important topic in the production of soybean. The quality of soybean proteins is limited by anti-nutrient proteins and low levels of essential sulfur amino acids. In this study, protein content and solubility of six cultivars were evaluated and seed storage proteins were analyzed using SDS-PAGE and scanning densitometry. The results showed that seed storage protein ba...
متن کامل